Resources

Eukaryotic Ribosome Biogenesis

Structure of an early small subunit assembly intermediate (SSU processome)

5.1 Å architectural model
PDB 5TZS, EMDB-8473

4.1 to 3.6 Å near-atomic model
PDB 5WLC, EMDB-8859

Pymol session

Chaker-Margot M., Barandun J., Hunziker M., S. Klinge. Architecture of the yeast small subunit processomeScience (2017); 355:eaal1880.

Barandun J.*, Chaker-Margot M.*, Hunziker M.*, Molloy K. R., Chait B. T. and S. Klinge. The complete structure of the small-subunit processomeNature Structural & Molecular Biology (2017) 24:944–53.

Barandun J.*, Hunziker M.*, Klinge S. Assembly and structure of the SSU processome – a nucleolar precursor of the small ribosomal subunit. Current Opinion in Structural Biology (2018) Jan;49 85-93.


Structure of an early large subunit assembly intermediate

State 2
PDB 6C0F, EMDB-7324

State 3
PDB 6CB1, EMDB-7445

Pymol session

Sanghai Z. A.*, Miller L.*, Molloy K. R., Barandun J., Hunziker M., Chaker-Margot M., Wang J., Chait B. T. & Klinge S. Modular assembly of the nucleolar pre-60S ribosomal subunitNature (2018) 556:126–9.


Pupylation – a prokaryotic protein modification

Molecular basis of pupylation – a prokaryotic ubiquitin-like protein modification system

Structure of the ligase PafA
PDB 4B0T

Structure of the ligase PafA
in complex with Pup
PDB 4BJR

Barandun J., Delley C. L., Ban N. & Weber-Ban E. Crystal structure of the complex between Prokaryotic Ubiquitin-like Protein Pup and its Ligase PafAJ. Am. Chem. Soc. (2013) 135: 6794–7.

Özcelik D.*, Barandun J.*, Schmitz N., Sutter M., Guth E., Damberger F. F., Allain F. H.-T., Ban N., Weber-Ban E. Structures of Pup ligase PafA and depupylase Dop from the prokaryotic ubiquitin-like modification pathwayNature Communications (2012) 3, 1014.